Monoclonal antibodies specific for elongation factor Tu and complete nucleotide sequence of the tuf gene in Mycobacterium tuberculosis
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چکیده
منابع مشابه
Nucleotide sequence of Mycobacterium leprae elongation factor (EF-Tu) gene.
The elongation factor EF-Tu is essential in bacterial translation and has sequences which are highly conserved even in phylogenetically distant bacteria. This allowed us to show that Gram negative bacteria had two copies of the tuf gene whereas most Gram positive bacteria including Mycobacteria had one copy of this gene (1). The agent of leprosy, Mycobacterium leprae, has been isolated from nat...
متن کاملThe amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence.
The complete amino acid sequence of elongation factor Tu of Escherichia coli has been established by sequencing overlapping cyanogen bromide and tryptic peptides. Sequence analysis of peptides was done primarily by solid-phase Edman degradation. Elongation factor Tu is a single chain polypeptide composed of 393 amino acids (Mr = 43,225). Its NH2 terminus is blocked with an acetyl group, as dete...
متن کاملCloning and nucleotide sequence of a tobacco chloroplast translational elongation factor, EF-Tu.
Translational elongation factor is an essential component for protein synthesis that functions by binding aminoacylated tRNAs to the ribosome-mRNA complex. This function is performed by EF-Tu in prokaryotes and eukaryotic organelles, and by EF-la in eukaryotes (for review see Miller and Weissbach, 1977). In lower photosynthetic eukaryotes, including Chlamydomonas (Baldauf and Palmer, 1990) and ...
متن کاملManipulation of the tuf gene provides clues to the localization of sequence element(s) involved in the thermal stability of Thermotoga maritima elongation factor Tu.
Truncated versions of the tuf gene for elongation factor Tu (EF-Tu; 400 aa) from the hyperthermophilic bacterium Thermotoga maritima have been produced by progressive 3'-->5' trimming. The truncated genes have been expressed in Escherichia coli and the thermal stability of the gene products has been assayed by monitoring their GDP-binding capacity after preheating the cell-free extracts at vari...
متن کاملInteraction of Mycobacterium tuberculosis elongation factor Tu with GTP is regulated by phosphorylation.
During protein synthesis, translation elongation factor Tu (Ef-Tu) is responsible for the selection and binding of the cognate aminoacyl-tRNA to the acceptor site on the ribosome. The activity of Ef-Tu is dependent on its interaction with GTP. Posttranslational modifications, such as phosphorylation, are known to regulate the activity of Ef-Tu in several prokaryotes. Although a study of the Myc...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1992
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.60.8.3136-3142.1992